That, IMO, is why you need to do thorough testing of your configuration before taking it live. If you are not confident in your system being stable, then yes, you should absolutely not use the configuration on a single machine live.
However, the software on seperate machines may not work properly as well. It's not just that the 2 softwares conflict, it may be a conflict of the hardware with the software you are trying to use. IRQ conflicts as an example, are rare these days, but still a possability.
Personally, I have run several apps at an event, only because I've tested them together on the machine I intended to use them on, for hours, as I intended to use them at a gig, but at home, for both practice using everything tegether, and to build confidence in my setups configuration.
As an example of what was running... I've had an apache server running on my system for remote access control, OtsAV running video with remote access control enabled, Opera to access the apache server PHP pages requested, Light Jockey, and Autohotkey (various control scripts used to control Ots via an XKeys controller. If I weren't running video, then I was running a milkdrop plugin for visual effects.
The last few events I've done, have not been as aggressive, as I no longer use the apache server. I've replaced it with a script that creates a gui, and accesses the Remote access control in Ots to query and display info directly. It's a bit easier on resources, and just as stable IMO. I also don't use milkdrop any longer, opting for the built in image slideshow if video isn't present. I am confident in my system. Are you of yours is the question you need to answer.