I have a friend who is a DJ and he now does not say "ladies and gentlemen" anymore after a guest got angry with him saying it was offensive to people who did not fall into either category. He now uses "hello everyone".
There are a bunch of these language changes that some people may or may not adopt as they go along.
Years ago I stopped calling it a "Bridal Party." When referring to the collection of bridesmaids and groomsmen. I now use the term "Wedding Party" so that I don't ever have to swap out Bridal party on the fly and have trouble removing it because it's built into a habit.
Similarly, to say something like "Good evening family and friends, it's a pleasure to be with you all to celebrate..." sounds very genuine. I don't think most groups are really going to get wound up over "Ladies and gentlemen," but there are plenty of ways to address the room when acting as the MC for an event.